Anaphylaxis increases 8-iso-prostaglandin F2alpha release from guinea-pig lung in vitro.
Eur J Pharmacol
; 365(1): 59-64, 1999 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-9988124
ABSTRACT
8-Iso-prostaglandin F2alpha, release from isolated and perfused guinea-pig lung was measured by radioimmunoassay. 8-Iso-prostaglandin F2alpha release was detectable under basal conditions and increased 10-fold during antigen-induced bronchoconstriction, concomitant with the increase of thromboxane B2 and prostaglandin E2. The anti-8-iso-prostaglandin F2alpha serum used in the radioimmunoassay seems to be quite specific for this compound. Pretreatment of lungs with indomethacin (a non-selective inhibitor of cyclooxygenase) reduced 8-iso-prostaglandin F2alpha release under basal conditions and completely abolished the increase observed during lung anaphylaxis. Pretreatment of lungs with NS 398 (N-[2-cyclohexyl]-4-nitrophenyl methanesulphonamide), a selective inhibitor of cyclooxygenase-2, did not change basal or antigen-induced 8-iso-prostaglandin F2alpha release at all. We conclude that under basal conditions guinea-pig lung perfusates contain low levels of 8-iso-prostaglandin F2alpha-like immunoreactivity, which increase 10-fold during antigen-induced bronchoconstriction. This isoprostane seems to be derived from the cyclooxygenation of arachidonic acid via the constitutive form of cyclooxygenase.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dinoprost
/
Anafilaxia
/
Pulmón
Límite:
Animals
Idioma:
En
Revista:
Eur J Pharmacol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Italia