Predominance of neuronal mRNAs in individual Alzheimer's disease senile plaques.
Ann Neurol
; 45(2): 174-81, 1999 Feb.
Article
en En
| MEDLINE
| ID: mdl-9989619
ABSTRACT
The sequestration of RNA in Alzheimer's disease (AD) senile plaques (SPs) and the production of intraneuronal amyloid-beta peptides (Abeta) prompted analysis of the mRNA profile in single immunocytochemically identified SPs in sections of AD hippocampus. By using amplified RNA expression profiling, polymerase chain reaction, and in situ hybridization, we assessed the presence and abundance of 51 mRNAs that encode proteins implicated in the pathogenesis of AD. The mRNAs in SPs were compared with those in individual CA1 neurons and the surrounding neuropil of control subjects. The remarkable demonstration here, that neuronal mRNAs predominate in SPs, implies that these mRNAs are nonproteinaceous components of SPs, and, moreover, that mRNAs may interact with Abeta protein and that SPs form at sites where neurons degenerate in the AD brain.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Mensajero
/
Placa Amiloide
/
Enfermedad de Alzheimer
Límite:
Aged
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Ann Neurol
Año:
1999
Tipo del documento:
Article
País de afiliación:
Estados Unidos