Liquid and Frozen Storage of Agouti (Dasyprocta leporina) Semen Extended with UHT Milk, Unpasteurized Coconut Water, and Pasteurized Coconut Water
Veterinary medicine international
; 2011: [1-5], Sep. 2010. tab, graf
Article
en En
| MedCarib
| ID: med-17637
Biblioteca responsable:
TT5
ABSTRACT
This study evaluated the effects of semen extension and storage on forward progressive motility % (FPM%) in agouti semen. Three extenders were used; sterilized whole cow's milk (UHT Milk), unpasteurized (CW) and pasteurized coconut water (PCW), and diluted to 50, 100, 150, and 200 10(6) spermatozoa/ml. Experiment 1 200 ejaculates were extended for liquid storage at 5(∘)C and evaluated every day for 5 days to determine FPM% and its rate of deterioration. Experiment 2 150 ejaculates were extended for storage as frozen pellets in liquid nitrogen at -195(∘)C, thawed at 30(∘) to 70(∘)C for 20 to 50 seconds after 5 days and evaluated for FPM% and its rate of deterioration. Samples treated with UHT milk and storage at concentrations of 100 10(6) spermatozoa/ml produced the highest means for FPM% and the slowest rates of deterioration during Experiment 1. During Experiment 2 samples thawed at 30(∘)C for 20 seconds exhibited the highest means for FPM% (12.18 ñ 1.33%), 85% rate of deterioration. However, samples were incompletely thawed. This was attributed to the diameter of the frozen pellets which was 1 cm. It was concluded that the liquid storage method was better for short term storage.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MedCarib
Asunto principal:
Preservación de Semen
/
Motilidad Espermática
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Espermatozoides
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Trinidad y Tobago
/
Criopreservación
/
Sustitutos de la Leche Humana
Límite:
Animals
País/Región como asunto:
Caribe ingles
/
Trinidad y tobago
Idioma:
En
Revista:
Veterinary medicine international
Año:
2010
Tipo del documento:
Article