SARS-CoV-2 surveillance in untreated wastewater: detection of viral RNA in a low-resource community in Buenos Aires, Argentina
Rev Panam Salud Publica
; 45, oct. 2021
Article
en En
| PAHOIRIS
| ID: phr-54988
Biblioteca responsable:
US1.1
ABSTRACT
[ABSTRACT]. Objective. To measure SARS-CoV-2 RNA in sewage in a low-resource community in order to determine if it can be considered as an estimator of changes in the prevalence of COVID-19 in the population. Methods. In this descriptive observational study we collected samples of surface waters contaminated with sewage and optimized a method of purification of viral RNA using PEG concentration. We determined the amount of genetic material by quantitative real-time PCR using the CDC method for SARS-CoV-2 detection. Results. We quantified viral RNA in surface waters contaminated with sewage of a low resource community and determined that temporal trends of SARS-CoV-2 in wastewater samples mirrored trends in COVID-19 active cases. Conclusions. Measuring of SARS-CoV-2 RNA in sewage can be applied in low-resource communities without connection to sewers as an estimator of changes in the prevalence of COVID-19.
Texto completo:
1
Colección:
04-international_org
Base de datos:
PAHOIRIS
Asunto principal:
Argentina
/
Monitoreo del Ambiente
/
Aguas Residuales
/
SARS-CoV-2
/
COVID-19
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
/
Screening_studies
País/Región como asunto:
America do sul
/
Argentina
Idioma:
En
Año:
2021
Tipo del documento:
Article