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Distinct phenotype of SARS-CoV-2 Omicron BA.1 in human primary cells but no increased host range in cell lines of putative mammalian reservoir species
Manel Essaidi-Laziosi; Francisco Javier Perez Rodriguez; Catia Alvarez; Pascale Sattonnet-Roche; Giulia Torriani; Meriem Bekliz; Kenneth Adea; Matthias Lenk; Wolfgang Preiser; Tasnim Suliman; Marcel A Muller; Christian Drosten; Laurent Kaiser; Isabella Eckerle.
Afiliación
  • Manel Essaidi-Laziosi; University of Geneva
  • Francisco Javier Perez Rodriguez; University Hospital of Geneva
  • Catia Alvarez; University of Geneva
  • Pascale Sattonnet-Roche; University Hospital of Geneva
  • Giulia Torriani; University of Geneva
  • Meriem Bekliz; University of Geneva
  • Kenneth Adea; University of Geneva
  • Matthias Lenk; Friedrich-Loeffler-Institute
  • Wolfgang Preiser; Stellenbosch University - Tygerberg Campus: Stellenbosch University Faculty of Medicine and Health Sciences
  • Tasnim Suliman; University of the Western Cape, Cape Town, South Africa
  • Marcel A Muller; Charite Universitatsmedizin Berlin
  • Christian Drosten; Charite Universitatsmedizin
  • Laurent Kaiser; University Hospital of Geneva
  • Isabella Eckerle; University of Geneva
Preprint en En | PREPRINT-BIORXIV | ID: ppbiorxiv-510352
ABSTRACT
SARS-CoV-2s genetic plasticity has led to several variants of concern (VOCs). Here we studied replicative capacity for seven SARS-CoV-2 isolates (B.1, Alpha, Beta, Gamma, Delta, Zeta, and Omicron BA.1) in primary reconstituted airway epithelia (HAE) and lung-derived cell lines. Furthermore, to investigate the host range of Delta and Omicron compared to ancestral SARS-CoV-2, we assessed replication in 17 cell lines from 11 non-primate mammalian species, including bats, rodents, insectivores and carnivores. Only Omicrons phenotype differed in vitro, with rapid but short replication and efficient production of infectious virus in nasal HAEs, in contrast to other VOCs, but not in lung cell lines. No increased infection efficiency for other species was observed, but Delta and Omicron infection efficiency was increased in A549 cells. Notably replication in A549 and Calu3 cells was lower than in nasal HAE. Our results suggest better adaptation of VOCs towards humans, without an extended host range.
Licencia
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Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Idioma: En Año: 2022 Tipo del documento: Preprint
Texto completo: 1 Colección: 09-preprints Base de datos: PREPRINT-BIORXIV Idioma: En Año: 2022 Tipo del documento: Preprint