The regulation mechanism of HCBP6 mimic phosphorylation on triglyceride synthesis in hepatocytes / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences)
; (6): 502-507, 2021.
Article
en Zh
| WPRIM
| ID: wpr-1006681
Biblioteca responsable:
WPRO
ABSTRACT
【Objective】 To investigate the regulation of HCBP6 mimic phosphorylation on triglyceride synthesis in hepatocytes so as to provide a molecular target for the treatment of metabolism-associated fatty liver disease. 【Methods】 We used site-directed mutagenesis to mimic constitutive phosphorylation and dephosphorylation of HCBP6 Ser-10 and Ser-151. Oil red O staining and triglyceride content determination were used to detect triglyceride levels in hepatocytes. The expressions of SREBP1c, ACC1 and FASN were detected by qRT-PCR and Western blotting. The Dual-Luciferase Report Gene System was used to detect SREBP1c promoter activity. 【Results】 HCBP6 Ser-10 phosphorylation promoted triglyceride synthesis. HCBP6 Ser-10 phosphorylation upregulated the expressions of SREBP1c, ACC1and FASN genes; HCBP6 Ser-10 phosphorylation enhanced the SREBP1c promoter activity. 【Conclusion】 HCBP6 Ser-10 phosphorylation can significantly enhance the activity of the SREBP1c promoter, upregulate the SREBP1c-FASN signal pathway transduction, and promote the synthesis of triglycerides.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Xi'an Jiaotong University(Medical Sciences)
Año:
2021
Tipo del documento:
Article