Association of genetic variants of m6A binding protein with the risk of gastric cancer / 实用医学杂志

ABSTRACT

Objective To investigate the association between single nucleotide polymorphisms(SNPs)of YTHDF1 rs6011668,HNRNPA2B1 rs2070601 and rs76558212 with the risk of gastric cancer.Methods A total of 457 cases with gastric cancer and 525 healthy controls were collected.The candidate SNPs were genotyped using Hi-SNP genotyping methods by multiplex rounds of PCR and high-throughput sequencing;the association between the three SNPs with the risk of gastric cancer was analyzed by test and Logistic regression.Multifactorial logistic regression and Risk Score(RS)model was used to analyze the influence of environmental and genetic factors on the risk of gastric cancer.Results YTHDF1rs6011668 TT genotype carriers had 3.075 times higher risk of gastric cancer than CC genotype carriers(95%CI1.128～8.382,P = 0.028),and 2.961 times higher risk than CC/TC genotypes carriers(95%CI1.091～8.033,P = 0.033).Subgroups-analysis revealed that TT genotype mainly increased the risk of gastric cancer in non-tea drinkers,pickled food eaters and fried food eaters(P<0.05).In addition,TT genotype carriers had the increased risk of gastric cancer infiltration,lymph node metastasis,distal metastasis and intermediate to advanced stages(P<0.05).The RS of the case and control groups were calculated by combining environmental and genetic factors.The higher the RS score,the higher the risk of gastric cancer was found in the RS quartile groups.Compared with the RS<Q25 group,the risk of gastric cancer in the Q25<RS<Q50,Q50<RS<Q75 and RS≥Q75 groups was 3.090,9.731 and 19.949,respectively.Conclusion The YTHDF1 rs6011668 mutant genotype may be associated with an increased risk and clinical progression of gastric cancer,and combining environmental and genetic factors could better assess the risk of gastric cancer.