Shikonin Inhibits APC-mutant Colon Cancer via Wnt/β-catenin Signaling / 肿瘤防治研究
Cancer Research on Prevention and Treatment
; (12): 402-408, 2024.
Article
en Zh
| WPRIM
| ID: wpr-1032169
Biblioteca responsable:
WPRO
ABSTRACT
Objective To identify small molecule inhibitors of APC-mutant colon cancer and provide lead compounds for targeted therapy of colon cancer. Methods APC-mutant colon cancer cell lines that stably express 7*Tcf-GFP/SV40-Cherry (7TGC) dual fluorescence reporter system was constructed for small-molecule inhibitor screening. Cell viability, colony formation, EdU incorporation, and xenograft tumor assay were used to evaluate the inhibitory effect of these inhibitors on APC-mutant colon cancer in vitro and in vivo. Western blot and co-immunoprecipitation assays were used to explore the molecular mechanism. Results Four small molecules that inhibited Wnt activity in APC-mutant colon cancer cells were discovered. Shikonin exhibited significant inhibition of cell viability and proliferation while inducing apoptosis of APC-mutant colon cancer cells. Xenograft tumor assay demonstrated that shikonin significantly reduced tumor growth in vivo. Furthermore, Western blot and co-immunoprecipitation assays revealed that shikonin markedly decreased β-catenin levels. Conclusion Shikonin effectively inhibits Wnt activity and suppresses tumor growth in APC-mutant colon cancer.
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1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Cancer Research on Prevention and Treatment
Año:
2024
Tipo del documento:
Article