Generation and Immunity Testing of a Recombinant Adenovirus Expressing NcSRS2-NcGRA7 Fusion Protein of Bovine Neospora caninum
The Korean Journal of Parasitology
; : 247-253, 2013.
Article
en En
| WPRIM
| ID: wpr-103945
Biblioteca responsable:
WPRO
ABSTRACT
Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was 10(9)TCID50/ml. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-gamma and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Proteínas Recombinantes de Fusión
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Inmunoglobulina G
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Proteínas Fúngicas
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Portadores de Fármacos
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Vacunas Fúngicas
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Vacunas Sintéticas
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Adenoviridae
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Interleucina-4
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Interferón gamma
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Neospora
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Año:
2013
Tipo del documento:
Article