Identification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR
Journal of Veterinary Science
; : 167-173, 2013.
Article
en En
| WPRIM
| ID: wpr-104702
Biblioteca responsable:
WPRO
ABSTRACT
Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment sizes of approximately 200 to 1,500 bp. The eight fragments were expressed differentially in the treatment group but not in the control. The sequences of two bands were similar to those of genes associated with the inflammatory process and a band was related to repair and regeneration process. Another one was related with spermatogonia and the rest four were unknown. Additionally, amplicons corresponding to the full-length sequences of two inflammatory gene fragments were synthesized by rapid amplification of cDNA end PCR. One showed 99% similarity to the major histocompatibility complex class II dog leukocyte antigen-DR beta chain and the other was canis familiaris proteasome beta type 3. Results of the present study suggested that sterile gauze induced the differential expression of genes in the omentum involved in inflammation and healing process.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Epiplón
/
Vendajes
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Cicatrización de Heridas
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ARN Mensajero
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Datos de Secuencia Molecular
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Secuencia de Bases
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Antígenos de Histocompatibilidad Clase II
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Regulación de la Expresión Génica
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ADN Complementario
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2013
Tipo del documento:
Article