Identification of parasite DNA in common bile duct stones by PCR and DNA sequencing
The Korean Journal of Parasitology
; : 301-306, 2007.
Article
en En
| WPRIM
| ID: wpr-114841
Biblioteca responsable:
WPRO
ABSTRACT
We attempted to identify parasite DNA in the biliary stones of humans via PCR and DNA sequencing. Genomic DNA was isolated from each of 15 common bile duct (CBD) stones and 5 gallbladder (GB) stones. The patients who had the CBD stones suffered from cholangitis, and the patients with GB stones showed acute cholecystitis, respectively. The 28S and 18S rDNA genes were amplified successfully from 3 and/or 1 common bile duct stone samples, and then cloned and sequenced. The 28S and 18S rDNA sequences were highly conserved among isolates. Identity of the obtained 28S D1 rDNA with that of Clonorchis sinensis was higher than 97.6%, and identity of the 18S rDNA with that of other Ascarididae was 97.9%. Almost no intra-specific variations were detected in the 28S and 18S rDNA with the exception of a few nucleotide variations, i.e., substitution and deletion. These findings suggest that C. sinensis and Ascaris lumbricoides may be related with the biliary stone formation and development.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
ADN Ribosómico
/
Datos de Secuencia Molecular
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ARN Ribosómico 18S
/
ARN Ribosómico 28S
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Secuencia de Bases
/
Cálculos Biliares
/
Reacción en Cadena de la Polimerasa
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Alineación de Secuencia
/
Clonorchis sinensis
/
Ascaris lumbricoides
Tipo de estudio:
Diagnostic_studies
Límite:
Aged80
/
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
The Korean Journal of Parasitology
Año:
2007
Tipo del documento:
Article