Leukocyte Sequestration and Free Radical-Mediated Lung Injury in Ovine Cardiopulmonary bypass Using Membrane Oxygenator / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
; : 978-983, 1999.
Article
en Ko
| WPRIM
| ID: wpr-121947
Biblioteca responsable:
WPRO
ABSTRACT
BACKGROUND:
Complement activation with transpulmonary leukocyte sequestration is considered a main mediator leading to ischemia-reperfusion lung(I-R) injury. We studied the role of leukocytes in the formation of I-R injury in ovine cardiopulmonary bypass(CPB) model with a membrane oxygenator. MATERIAL ANDMETHOD:
Five sheep were used. CPB circuitry consisted of a roller pump(American Optical Corp., Greenwich, CT, USA) and a membrane oxygenator(UNIVOX-IC, Bentley, Baxter Health Corp, Irvine, CA, USA). The CPB time was fixed at 120 min. Ten minutes after the start of CPB, total CPB was established. Thereafter a total CPB of 100 min was performed, followed by another 10 min of partial CPB. The CPB was discontinued and the animals were fully recovered. For measuring left and right atrial leukocyte counts, blood samples were taken before thoracotomy, 5 min and 109 in after the start of CPB, and 30 min and 120 min after weaning. C3a was measured before thoracotomy, 109 min after the start of CPB, and 30 min and 120 min after weaning. Plasma malondialdehyde(MDA) was checked before thoracotomy, 109 min after the start of CPB, and 30 min after weaning. One to two grams of lung tissue were taken for water content measurement before thoracotomy, 109 min after the start of CPB, and 30 min after weaning. Lung biopsy specimens were examined by light and electron microscopy.RESULT:
Of 5 animals, 4 survived the experimental procedures. Of these, 3 animals survived on a long-term basis. No significant differences in transpulmonary gradients of leukocyte were found and no significant complement activation was expressed by C3a levels. MDA level did not show significant changes related to lung reperfusion despite an increase after the start of CPB. On both light and electron microscopic examinations, mild to moderate acute lung change was observed. Interstitial edema, leakage of erythrocytes into the alveolar space and endothelial cell swelling were the main findings. Water content of the lung showed a slight increase after the start of CPB, but there was no statistical significance.CONCLUSION:
These findings indicate that ischemia-repersusion lung injury may not be from complement activation-leukocyte sequestration but from another source of oxygen free radicals related to CPB.Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Preservación de Órganos
/
Oxígeno
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Oxigenadores de Membrana
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Plasma
/
Destete
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Biopsia
/
Proteínas del Sistema Complemento
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Ovinos
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Toracotomía
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Microscopía Electrónica
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Ko
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Año:
1999
Tipo del documento:
Article