Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry / 한국실험동물학회지
Laboratory Animal Research
; : 45-53, 2014.
Article
en En
| WPRIM
| ID: wpr-124667
Biblioteca responsable:
WPRO
ABSTRACT
Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential is hindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, the embryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5 protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerol lipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressed in neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alpha-fetoprotein and transferrins. Taken together, we found out that several proteins were significantly up- or down- regulated from pig pancreas based on developmental stage. This study will provide basis for understanding development of pig pancreas.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Páncreas
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Espectrometría de Masas
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Transferrina
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Quimotripsina
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Tripsina
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Electroforesis en Gel Bidimensional
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Alfa-Fetoproteínas
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Sus scrofa
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Parto
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Electroforesis
Límite:
Adult
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Humans
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Newborn
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Pregnancy
Idioma:
En
Revista:
Laboratory Animal Research
Año:
2014
Tipo del documento:
Article