Granulocyte Colony Stimulating Factor Attenuates Hyperoxia-Induced Lung Injury by Down-Modulating Inflammatory Responses in Neonatal Rats
Yonsei Medical Journal
; : 65-73, 2011.
Article
en En
| WPRIM
| ID: wpr-146144
Biblioteca responsable:
WPRO
ABSTRACT
PURPOSE:
Granulocyte colony stimulating factor (G-CSF) has been known to increase neutrophil production and have anti-inflammatory properties, but the effect of G-CSF on pulmonary system is in controversy. We investigated whether G-CSF treatment could attenuate hyperoxia-induced lung injury, and whether this protective effect is mediated by the down-modulation of inflammatory responses in a neonatal rat model. MATERIALS ANDMETHODS:
Newborn Sprague-Dawley rats (Orient Co., Seoul, Korea) were subjected to 14 days of hyperoxia (90% oxygen) beginning within 10 h after birth. G-CSF (20 microg/kg) was administered intraperitoneally on the fourth, fifth, and sixth postnatal days.RESULTS:
This treatment significantly improved hyperoxia-induced reduction in body weight gain and lung pathology such as increased mean linear intercept, mean alveolar volume, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling positive cells. Hyperoxia-induced activation of nicotinamide adenine dinucleotide phosphate oxidase, which is responsible for superoxide anion production, as evidenced by upregulation and membrane translocation of p67phox was significantly attenuated after G-CSF treatment, as were inflammatory responses such as increased myeloperoxidase activity and mRNA expression of transforming growth factor-beta. However, the attenuation of other proinflammatory cytokines such as tumor necrosis factor-alpha and interleukin-6 was not significant.CONCLUSION:
In sum, G-CSF treatment significantly attenuated hyperoxia-induced lung injury by down-modulating the inflammatory responses in neonatal rats.Palabras clave
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Aumento de Peso
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Distribución Aleatoria
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Western Blotting
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Factor Estimulante de Colonias de Granulocitos
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Factor de Crecimiento Transformador beta
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Interleucina-6
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Factor de Necrosis Tumoral alfa
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Ratas Sprague-Dawley
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Peroxidasa
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Hiperoxia
Tipo de estudio:
Prognostic_studies
Límite:
Animals
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Pregnancy
Idioma:
En
Revista:
Yonsei Medical Journal
Año:
2011
Tipo del documento:
Article