Negative-pressure wound therapy induces endothelial progenitor cell mobilization in diabetic patients with foot infection or skin defects
Experimental & Molecular Medicine
; : e62-2013.
Article
en En
| WPRIM
| ID: wpr-152457
Biblioteca responsable:
WPRO
ABSTRACT
Non healing chronic wounds are difficult to treat in patients with diabetes and can result in severe medical problems for these patients and for society. Negative-pressure wound therapy (NPWT) has been adopted to treat intractable chronic wounds and has been reported to be effective. However, the mechanisms underlying the effects of this treatment have not been elucidated. To assess the vasculogenic effect of NPWT, we evaluated the systemic mobilization of endothelial progenitor cells (EPCs) during NPWT. Twenty-two of 29 consecutive patients who presented at the clinic of Seoul National Universty Hospital between December 2009 and November 2010 who underwent NPWT for diabetic foot infections or skin ulcers were included in this study. Peripheral blood samples were taken before NPWT (pre-NPWT) and 7-14 days after the initiation of NPWT (during-NPWT). Fluorescence-activated cell sorting (FACS) analysis showed that the number of cells in EPC-enriched fractions increased after NPWT, and the numbers of EPC colony forming units (CFUs) significantly increased during NPWT. We believe that NPWT is useful for treating patients with diabetic foot infections and skin ulcers, especially when these conditions are accompanied by peripheral arterial insufficiency. The systemic mobilization of EPCs during NPWT may be a mechanism for healing intractable wounds in diabetic patients with foot infections or skin defects via the formation of increased granulation tissue with numerous small blood vessels.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Células Madre
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Endotelio Vascular
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Estudios de Casos y Controles
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Citocinas
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Ensayo de Unidades Formadoras de Colonias
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Pie Diabético
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Células Endoteliales
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Terapia de Presión Negativa para Heridas
Tipo de estudio:
Observational_studies
Límite:
Adolescent
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Adult
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Aged
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Aged80
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Child
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Female
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Humans
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Male
Idioma:
En
Revista:
Experimental & Molecular Medicine
Año:
2013
Tipo del documento:
Article