Construction of vectors expressing M2 and NA genes of H5N1 influenza virus / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 167-169, 2011.
Article
en Zh
| WPRIM
| ID: wpr-231161
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct vectors expressing M2 and NA genes of H5N1 influenza virus.</p><p><b>METHODS</b>Based on the human H5N1 avian influenza virus (A/Anhui/1/2005) isolated in china, M2 and NA genes were amplified by PCR. M2 or NA gene was subcloned into pStar vector to construct recombinant pStar-M2/, pStar-/M2, pStar-NA/and pStar-NA/. Furthermore, both of the M2 and NA genes were subcloned into pStar to construct two genes co-expressing recombinant pStar-M2/NA and pStar-NA/M2. Expression of the genes were detected by IFA after transfection of 293 cells with the recombinant plasmids.</p><p><b>RESULTS</b>Recombinant plasmids were constructed and identified by restriction endonuclease digestion. Expression of the genes cloned into the recombinant plasmids was confirmed by IFA.</p><p><b>CONCLUSION</b>Recombinant plasmids expressing M2 and/or NA genes of H5N1 influenza virus were constructed, which provided basis for development of influenza DNA vaccine.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plásmidos
/
Proteínas Virales
/
Línea Celular
/
Proteínas de la Matriz Viral
/
Subtipo H5N1 del Virus de la Influenza A
/
Vectores Genéticos
/
Genética
/
Metabolismo
/
Neuraminidasa
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2011
Tipo del documento:
Article