Experimental study of adipose-derived mesenchymal stem cells in the treatment of Crohn's disease / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 58-64, 2015.
Article
en Zh
| WPRIM
| ID: wpr-234958
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the efficacy of adipose-derived mesenchymal stem cells (ADMSCs) in a murine model of inflammatory bowel disease, and its potential mechanism.</p><p><b>METHODS</b>Murine colitis mouse model of Crohn's disease(CD) was created by trinitrobenzene sulfonic acid(TNBS)-induced colitis. Seventy-five 6-8 weeks female BALB/c mice were randomly divided into 3 groups: control group, TNBS group and ADMSC group. To verify the therapeutic effect of ADMSC, real-time PCR and immunohistochemical staining were performed to measure inflammatory cytokines levels in colon tissues. The 10-day survival statuses were recorded after the infusion of ADMSCs.</p><p><b>RESULTS</b>Intraperitoneal injection of ADMSCs alleviated the clinical and histopathologic severity of intestinal inflammation, and increased survival(60% vs. 30%, P<0.05) in the TNBS-induced mouse model of CD. Compared with TNBS group, proinflammatory cytokines, including TNF-α, IL-12 and VEGF of ADMSC group were significantly reduced, with significant increase of IL-10 expression.</p><p><b>CONCLUSION</b>ADMSCs can effectively repair the injury of colonitis through down-regulation of proinflammatory cytokines TNF-α, IL-12 and VEGF expression, and up-regulation of anti-inflammatory cytokine IL-10 expression, which may be a potential new alternative of cell-based therapy for CD.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Ácido Trinitrobencenosulfónico
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Enfermedades Inflamatorias del Intestino
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Enfermedad de Crohn
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Regulación hacia Abajo
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Regulación hacia Arriba
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Citocinas
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Colitis
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Adipocitos
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Modelos Animales de Enfermedad
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Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2015
Tipo del documento:
Article