Study on urine biomarkers in 1,3-butadiene exposed workers / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the urine biomarkers in 1,3-butadiene exposed workers, and to provide basement for establishing biological limit value.</p><p><b>METHODS</b>44 BD exposed workers as exposure group and 25 BD non-exposed people as control group including 12 workers in boiler workshop in the same factory and 13 people in one public institute, we collected their in-end-of shift urine, then detected urine BD-derived mercapturic metabolites [3,4-dihydroxybutyl mercapturic acid (DHBMA),1- and 2-monohydroxy-3-butenyl mercapturic acid (MHBMA)] concentrations using UPLC-MS/MS method. Meanwhile, we detected air BD concentration with GC-FID in the workplace, and compared their relationship.</p><p><b>RESULTS</b>lgDHBMA and lg (MHBMA + DHBMA) levels in exposed group (lgDHBMA 2.51 ± 0.44) µg/L, lg [MHBMA + DHBMA (2.68 ± 0.27) µg/L] were higher than which in control group (lgDHBMA (2.20 ± 0.25) µg/L, lg(MHBMA + DHBMA (2.49 ± 0.34) µg/L), and the differences were significant (P < 0.01). Urine DHBMA was obviously influenced by air BD concentrations (r = 0.539, P = 0.001). The equation of Multiple Regression Analysis was y = 2.417 + 0.520x (x represents air BD dose, and represents urinary DHBMA level). Adjusted R(2) of this model was 0.262. Urinary MHBMA was not affected by smoking, alcohol and years of works.</p><p><b>CONCLUSION</b>Urine metabolite DHBMA in BD-exposed workers might be major biological exposure indice.</p>