Effect of Meisoindigo on Wnt signal pathway in K562 and HL-60 cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 579-582, 2010.
Article
en Zh
| WPRIM
| ID: wpr-243309
Biblioteca responsable:
WPRO
ABSTRACT
The aim of this study was to investigate the effect of meisoindigo on Wnt signal pathway in K562 cells and HL-60 cells and its possible regulatory mechanism. RT-PCR and Western blot assay were used to detect the expression of GSK-3beta and its downstream associated genes as well as proteins expression respectively. The results showed that the meisoindigo could inhibit the phosphorylation of GSK-3beta and decreased beta-catenin and c-myc genes expression in HL-60 cells, but did not significantly affect the two gene expression in K562 cells. Meisoindigo slightly increased the expression of GSK-3beta protein in HL-60 cells, obviously decreased the expression of p-GSK-3beta and c-MYC proteins in K562 cells and HL-60 cells, while meisoindigo did not affect the expression of beta-catenin in K562 cells significantly, but could decrease the expression of beta-catenin in HL-60 cells. It is concluded that the meisoindigo can affect the Wnt signal pathway through inhibiting the GSK-3beta expression and down-regulating the beta-catenin and c-MYC protein expression, which play an important role in the treatment for chronic myeloid leukemia.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Transducción de Señal
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Proteínas Proto-Oncogénicas c-myc
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Células HL-60
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Células K562
/
Glucógeno Sintasa Quinasa 3
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Proteínas Wnt
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Beta Catenina
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Glucógeno Sintasa Quinasa 3 beta
/
Indoles
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2010
Tipo del documento:
Article