Study on polymethacrylate nanoparticles as delivery system of antisense oligodeoxynucleotides / 药学学报
Acta Pharmaceutica Sinica
; (12): 298-301, 2003.
Article
en Zh
| WPRIM
| ID: wpr-251119
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the possibility of polymethacrylate nanoparticles (NP) for antisense oligodeoxynucleotides delivery system.</p><p><b>METHODS</b>The nanoparticles were prepared by evaporating ethenol solution containing Eudragit RL100 or RS100, and then mixtured with oligonucleotides. The morphology and size were investigated by a transmission electron microscope and Mastersizer particle characterization systems, and the cytotoxicity was evaluated by Trypan Blue staining and hemolysis test. The flow cytometer was used to determine the uptake of fluorescence-labelled oligodeoxynucleotides.</p><p><b>RESULTS</b>The morphology of nanoparticles showed spherical and orderly, the average diameter was about 127 nm, and almost the antisense oligodeoxynucleotides (ODN) were loaded when NP ODN was 6.6. The uptake of ODN was significantly increased when loaded by nanoparticles, which well depended on the nanoparticles concentration. Meanwhile, slightly cytotoxicity was observed when high dose of nanoparticles was used.</p><p><b>CONCLUSION</b>The polymethacrylate nanoparticles appeared to be a promising vehicle for gene delivery.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Tamaño de la Partícula
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Resinas Acrílicas
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Portadores de Fármacos
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Química
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Oligonucleótidos Antisentido
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Tecnología Farmacéutica
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Sistemas de Liberación de Medicamentos
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Nanotecnología
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Toxicidad
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Hemólisis
Límite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2003
Tipo del documento:
Article