Effect of MK801 on apoptosis in the development of brain injury after hind limbs ischemia/reperfusion in rats / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 276-279, 2007.
Article
en Zh
| WPRIM
| ID: wpr-253424
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To evaluate development of brain injury after hind limbs ischemia/reperfusion (LI/R) in rats, and the effect of MK801 on the brain injury following LI/R.</p><p><b>METHODS</b>The limbs ischemia/reperfusion model was established in rats. The MDA contents were evaluated in each group, apoptotic cells were detected with TUNEL, the expression of apoptosis-associated protein, such as bcl-2, cytoC and caspase-3 were determined with immunohistochemistry and Western-blot.</p><p><b>RESULTS</b>The contents of MDA in brain tissue increased significantly following LI/R. The expression of bcl-2, cytoC, Caspase-3 was increased than those in the control group (P < 0.01) following LI/R significantly. The expression of Caspase-3 was increased 24 h after the onset of reperfusion. The expression of Caspase-3, bcl-2 gene was quite obvious in the midbrain red nucleus region. MK801 inhibited the expression of bcl-2, cytoC, Caspase-3 obviously.</p><p><b>CONCLUSION</b>The excessive apoptosis and apoptosis-associated factors could play an important role in the brain injury following LI/R in rat, MK801 might decrease the production of free radical and the excite toxicity of glutamate, inhibit the expression of apoptosis associated protein and reduce the occurrence of apoptosis.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Lesiones Encefálicas
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Daño por Reperfusión
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Maleato de Dizocilpina
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Ratas Sprague-Dawley
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Apoptosis
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Proteínas Proto-Oncogénicas c-bcl-2
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Citocromos c
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Caspasa 3
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Applied Physiology
Año:
2007
Tipo del documento:
Article