The cytoskeleton and immigration of laryngeal squamous carcinoma cell affected by Fascin-1 expression / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 1034-1038, 2010.
Article
en Zh
| WPRIM
| ID: wpr-260944
Biblioteca responsable:
WPRO
ABSTRACT
This study sought to detect the effect of Fascin-1 expression on the cytoskeleton and immigration of laryngeal squamous carcinoma cell. In the experiment, Fascin-1 expression in Hep-2 cells was inhibited by small interfering RNA. The cytoskeleton of Hep-2 cells was observed with the use of laser scanning confocal fluorescence microscope. Millicell insert was applied to detect the immigration of Hep-2 cells in vitro. The results showed that the integrity of cytoskeleton in Hep-2 cells was broken with the down-regulation of Fascin-1 expression and the immigration ability was decreased significantly (P < 0.05). The inhibiting ratio of cell immigration was 44.6 +/- 6.3%. In conclusion, inhibition of Fascin-1 expression in Hep-2 cells could break the integrity of cytoskeleton and decrease the ability of cellular immigration.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Citoesqueleto
/
Carcinoma de Células Escamosas
/
Proteínas Portadoras
/
Regulación hacia Abajo
/
Movimiento Celular
/
Neoplasias Laríngeas
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ARN Interferente Pequeño
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Línea Celular Tumoral
/
Genética
/
Metabolismo
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2010
Tipo del documento:
Article