Expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells in children with bronchial asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
; (12): 614-617, 2016.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-261180
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression profiles of PI3K, NF-κB, and STAT1 in peripheral blood mononuclear cells (PBMCs) in children with bronchial asthma, as well as their roles in the pathogenesis of asthma.</p><p><b>METHODS</b>Thirty children with acute exacerbation of bronchial asthma were enrolled as the asthma group, and 20 healthy children were enrolled as the control group. RT-PCR and Western blot were used to measure the mRNA and protein expression levels of PI3K, NF-κB, and STAT1 in PBMCs. A spirometer was used to compare the pulmonary function between the two groups. The correlations between the mRNA expression of PI3K, NF-κB, and STAT1 and pulmonary function in children with bronchial asthma were analyzed.</p><p><b>RESULTS</b>The asthma group had significantly higher mRNA and protein expression levels of PI3K, NF-κB, and STAT1 than the control group (P<0.05). Compared with the control group, the asthma group showed significant reductions in pulmonary function indices such as FEV1%, FEV1/FVC, and PEF% (P<0.05). In children with bronchial asthma, the mRNA expression levels of PI3K, NF-κB, and STAT1 were negatively correlated with FEV1%, FEV1/FVC, and PEF% (P<0.05).</p><p><b>CONCLUSIONS</b>The expression levels of PI3K, NF-κB, and STAT1 increase in children with asthma, and are negatively correlated with pulmonary function indices, suggesting that PI3K, NF-κB and STAT1 are involved in the development and progression of bronchial asthma in children.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Asma
/
Sangre
/
ARN Mensajero
/
Leucocitos Mononucleares
/
Química
/
Volumen Espiratorio Forzado
/
FN-kappa B
/
Fosfatidilinositol 3-Quinasas
/
Factor de Transcripción STAT1
Límite:
Niño
/
Niño, preescolar
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2016
Tipo del documento:
Artículo