Detection of TERC gene amplification by fluorescence in-situ hybridization in cervical intraepithelial lesions / 中华病理学杂志
Chinese Journal of Pathology
; (12): 182-186, 2011.
Article
en Zh
| WPRIM
| ID: wpr-261832
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the feasibility and practical value of fluorescence in situ hybridization (FISH) detection of TERC gene amplification in cervical intraepithelial lesions (CIN) and squamous cell carcinoma (SCC).</p><p><b>METHODS</b>Tissue microarray was constructed to cover 150 cases of various cervical conditions, including 24 cases of normal cervical mucosa, 78 cases of CINs (CINI, 25 cases; CINII, 21 cases and CINIII, 32 cases) and 48 cases of SCC. FISH was used to detect TERC gene amplification.</p><p><b>RESULTS</b>TERC gene amplification was detected in 8% (2/25) CINI, 47.6% (10/21) CINII, 71.9% (23/32) CINIII and 87.5% (42/48) SCC. There were significant differences among these groups (P < 0.05). The amplification rates of TERC gene in SCC, CINIII and CINII were significantly higher than those of normal cervical epithelium and CINI (P < 0.05). Significant differences were also observed among CINI and CINII, CINIII and SCC (P < 0.05), and between CINII and SCC (P < 0.05). There were no significant differences between normal cervical epithelium and CINI, CINII and CIN III, and between CINIII and SCC (P > 0.05). FISH detection of amplification of TERC gene in CINI and CINII-III demonstrated the following statistics sensitivity of 62.3%, specificity of 92.0%, accuracy of 71.8%, positive and negative predictive values of 94.3% and 53.5%, respectively.</p><p><b>CONCLUSIONS</b>FISH detection is a reliable method in detecting TERC gene amplification using paraffin tissue sections. When histological evaluation becomes difficult, TERC amplification detectable by FISH may offer a diagnostic distinction of CINI from CINII. Moreover, TERC amplification may be used as a biomarker in predicting CIN progression to invasive cancer.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
ARN
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Carcinoma de Células Escamosas
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Biomarcadores de Tumor
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Adenoma
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Displasia del Cuello del Útero
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Neoplasias del Cuello Uterino
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Amplificación de Genes
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Sensibilidad y Especificidad
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Hibridación Fluorescente in Situ
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Progresión de la Enfermedad
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Aged
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Pathology
Año:
2011
Tipo del documento:
Article