Effect of bortezomib and low concentration cytarabine on apoptosis in U937 cell line / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 554-557, 2012.
Article
en Zh
| WPRIM
| ID: wpr-263351
Biblioteca responsable:
WPRO
ABSTRACT
This study was aimed to explore the effect of bortezomib and low concentration cytarabine (Ara-C) on proliferation and apoptosis in U937 cell line and its mechanism. The proliferation and apoptosis of U937 cells treated with bortezomib (10 nmol/L) and(or) Ara-C (50 nmol/L) were observed by cell count, cell morphology, flow cytometry and Western blot. The results showed that bortezomib and Ara-C alone inhibited U937 cell proliferation. The inhibitory effect was enhanced by combination of these two drugs, the inhibitory rates of U937 cell proliferation were (55.00 ± 2.81)% and (70.02 ± 3.33)% after treatment for 24 h and 48 h, respectively. Bortezomib and Ara-C synergistically induced apoptosis and decreased mitochondrial membrane potential in U937 cells. The percentage of Rhodamin123 positive cells was (38.70 ± 1.54)%. Bortezomib and Ara-C also synergistically induced activation of caspase-9, caspase-8 and caspase-3. It is concluded that the bortezomib and low concentration Ara-C synergistically induced apoptosis in U937 cells, mainly through mitochondrial pathway, and possibly through death receptor pathway.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
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Pirazinas
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Ácidos Borónicos
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Ciclo Celular
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Apoptosis
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Células U937
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Citarabina
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Bortezomib
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2012
Tipo del documento:
Article