Cysteinyl leukotriene receptor 1 antagonist pranlukast modulates differentiation of SK-N-SH cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 123-128, 2007.
Article
en Zh
| WPRIM
| ID: wpr-271564
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether cysteinyl leukotriene receptor agonist LTD(4) and cysteinyl leukotriene receptor 1 (CysLT(1)) antagonist pranlukast affect the differentiation of human neuroblastoma SK-N-SH cells.</p><p><b>METHODS</b>SK-N-SH cell morphological changes induced by LTD(4), pranlukast and LTD(4) + pranlukast were observed with retinoid acid (RA) as the positive control. The expressions of CysLT(1) and CysLT(2) receptors were detected by immunoblotting analysis, and the expression of microtubule-associated protein-2 (MAP-2), a neuron marker, was detected by fluorescent immunostaining.</p><p><b>RESULT</b>The immunoblotting results showed that SK-N-SH cells expressed CysLT(1) receptor moderately, and CysLT(2) receptor highly. The morphological results showed that RA, pranlukast and LTD(4) + pranlukast induced the compaction of the cell bodies and the outgrowth of neurites, while LTD(4) had no significant effect. The immunostaining results showed that MAP-2 was distributed in the cell bodies in control or pranlukast-treated cells; it was distributed in cell bodies and neuritis in RA-treated cells. Pranlukast increased the numbers of MAP-2-positive cells.</p><p><b>CONCLUSION</b>The CysLT(1)receptor antagonist pranlukast modulates the differentiation of SK-N-SH cells.</p>
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Asunto principal:
Patología
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Farmacología
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Inmunohistoquímica
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Immunoblotting
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Diferenciación Celular
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Cromonas
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Receptores de Leucotrienos
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Leucotrieno D4
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Antagonistas de Leucotrieno
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Línea Celular Tumoral
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2007
Tipo del documento:
Article