Dynamic observation of lung metastasis model of adenoid cystic carcinoma / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To form ACC-M-GFP cells by transfecting pEGFP-1 into ACC-M cells, and to build up lung metastasis model of adenoid cystic carcinoma for dynamic observation in simulated lung environment.</p><p><b>METHODS</b>pEGFP-1 was prepared and then transfected into ACC-M cell lines by using cationic lipid-based gene transfer technique. After successive selection, the ACC-M-GFP cells were collected and inoculated in BALB/C mice. The simulated lung environment was built up, and dynamic observation was performed by laser confocal microscopy within four weeks after transfection.</p><p><b>RESULTS</b>The amount of fluorescent tumor cell colonies and the intensity of fluorescence gradually increased from the second week to the forth week after transfection. In the meantime, only a very small number of tumor cells had the ability to form clones.</p><p><b>CONCLUSIONS</b>We successfully built up a lung metastasis model of adenoid cystic carcinoma for dynamic observation. The model is suitable for capturing and analyzing metastatic tumor cells in early stage.</p>