Study of biological safety of scaffold material with heterologous deproteinized bone / 中华创伤杂志(英文版)

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications.</p><p><b>METHODS</b>Deproteinized bone (10 mm) and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test.</p><p><b>RESULTS</b>No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramuscular implantation and cytotoxity were observed.</p><p><b>CONCLUSIONS</b>The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering.</p>