Toxic effect of butenolide on chondrocyte differentiation and the protective effect of selenium / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 382-385, 2006.
Article
en Zh
| WPRIM
| ID: wpr-281192
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of butenolide (BUT) on cultured chondrocytes differentiation and the possible protective effects of selenium (Se).</p><p><b>METHODS</b>Ex-vivo cultured chondrocytes were divided into six groups: (1) Control group (without BUT and Se); (2) Se 0.1 microg/ml control group; (3) BUT 0.1 microg/ml group; (4) BUT 1.0 microg/ml group; (5) BUT 5.0 microg/ml group; and (6) BUT 1.0 microg/ml + Se 0.1 microg/ml group. The expression of collagen II (Col II), collagen X (ColX), basic fibroblast growth factor (bFGF), and parathyroid hormone-related peptide (PTHrP) in (or around) chondrocytes in all groups were analyzed by immunohistochemistry.</p><p><b>RESULTS</b>The expressions of Col II in 1.0 microg/ml BUT group and 5.0 microg/ml BUT group were significantly lower than those in the control group (P < 0.05). The expression of Col II in 1.0 microg/ml BUT + Se group were significantly higher than those in the 1.0 microg/ml BUT group and 5.0 microg/ml BUT group (P < 0.05). The expressions of bFGF and PTHrP of BUT groups were significantly higher than those in the Se and control groups (P < 0.05). No expression of ColX was observed in all groups.</p><p><b>CONCLUSION</b>BUT can affect the collagen II synthesis of the chondrocytes. Selenium supplementation may play a protective role.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Selenio
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Toxina T-2
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4-Butirolactona
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Diferenciación Celular
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Células Cultivadas
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Condrocitos
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Sustancias Protectoras
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Biología Celular
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Toxicidad
Límite:
Humans
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Año:
2006
Tipo del documento:
Article