Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p><p><b>METHOD</b>The analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.</p><p><b>RESULT</b>The calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.</p><p><b>CONCLUSION</b>The results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.</p>