microRNA-21 and microRNA-30b expression in multiple myeloma / 中华血液学杂志
Chinese Journal of Hematology
; (12): 38-41, 2010.
Article
en Zh
| WPRIM
| ID: wpr-283889
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of miR-21 and miR-30b in multiple myeloma (MM).</p><p><b>METHODS</b>Peripheral blood mononuclear cells from patients with MM were cultured at 2.5 x 10(6) cells/ml in alpha-MEM supplemented with 10% of fetal bovine serum, antibiotics, RANKL (50 ng/ml), and macrophage colony-stimulating factor (25 ng/ml) for 10 to 14 days to obtain osteoclasts with bone-resorbing activity. Primary myeloma cells were purified from 12 MM patients. Of them, 8 samples were cocultured with osteoclasts and 4 as noncocultured control. The expression of miR-21 and miR-30b was detected by real-time PCR.</p><p><b>RESULTS</b>The viability of MM cells recovered from cocultures was higher than those of noncocultured control. After cocultured with osteoclasts, primary myeloma cells from eight patients exhibited a 1.3- 5.9-fold increase in miR-21 expression and 1.38- 4.32-fold decrease in miR-30b expression compared with controls. In highly purified plasma cells from 3 healthy subjects, 12 MM patients and 11 MM cell lines, the expression of miR-21 was 1.9 +/- 0.8, 6.5 +/- 4.9 and 35.1 +/- 36.2, respectively; the expression of miR-30b was 13.6 +/- 1.8, 7.2 +/- 6.3 and 4.5 +/- 1.9, respectively.</p><p><b>CONCLUSIONS</b>miR-21 acts as an oncogene and miR-30b a tumor suppressor gene in MM.</p>
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Base de datos:
WPRIM
Asunto principal:
Leucocitos Mononucleares
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MicroARNs
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Línea Celular Tumoral
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Reacción en Cadena en Tiempo Real de la Polimerasa
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Genética
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Metabolismo
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Mieloma Múltiple
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Año:
2010
Tipo del documento:
Article
Pais de publicación:
CHINA
/
CN
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REPUBLIC OF CHINA