Role of axl in preeclamptic EPCs functions / 华中科技大学学报(医学)(英德文版)
Journal of Huazhong University of Science and Technology (Medical Sciences)
; (6): 395-401, 2016.
Article
en En
| WPRIM
| ID: wpr-285256
Biblioteca responsable:
WPRO
ABSTRACT
Axl encodes the tyrosine-protein kinase receptor, participating in the proliferation and migration of many cells. This study examined the role of Axl in functions of endothelial progenitor cells (EPCs). Axl was detected by RT-PCR and Western blotting in both placentas and EPCs from normal pregnancy and preeclampsia patients. The Axl inhibitor, BMS777-607, was used to inhibit the Axl signalling pathway in EPCs. Cell proliferation, differentiation, migration and adhesion were measured by CCK-8 assay, cell differentiation assay, Transwell assay, and cell adhesion assay, respectively. Results showed the expression levels of Axl mRNA and protein were significantly higher in both placentas and EPCs from preeclampsia patients than from normal pregnancy (P<0.05). After treatment with BMS777-607, proliferation, differentiation, migration and adhesion capability of EPCs were all significantly decreased. Our study suggests Axl may play a role in the function of EPCs, thereby involving in the pathogenesis of preeclampsia.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Placenta
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Preeclampsia
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Piridonas
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Células Madre
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Sangre
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Presión Sanguínea
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ARN Mensajero
/
Estudios de Casos y Controles
Tipo de estudio:
Observational_studies
Límite:
Adult
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Female
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Humans
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Pregnancy
Idioma:
En
Revista:
Journal of Huazhong University of Science and Technology (Medical Sciences)
Año:
2016
Tipo del documento:
Article
Pais de publicación:
CHINA
/
CN
/
REPUBLIC OF CHINA