Osteopontin promotes the malignant phenotypes of human hepatocellular carcinoma cell line SMMC-7721 / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 37-40, 2007.
Article
en Zh
| WPRIM
| ID: wpr-285485
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of osteopontin (OPN) on the phenotypes of human hepatocellular carcinoma cell line SMMC-7721.</p><p><b>METHODS</b>Human hepatocellular carcinoma SMMC-7721 cells were transfected with plasmid pcDNA 3.1(-)/OPN and cells transfected with a mock plasmid served as controls. OPN expression was verified by RT-PCR and Western blot, and concentrations of OPN, MMP-2, MMP-9 and uPA were measured by ELISA. A series of functional assays in vitro were used to monitor the changes of SMMC-7721 malignant phenotypes.</p><p><b>RESULTS</b>OPN expression of SMMC-7721 cells was elevated after transfection. Concentrations of OPN, MMP-2 and uPA in the medium of SMMC-7721 cells after transfection were higher than those of the controls [(3.02+/-0.12) ng/ml vs (1.43+/-0.07) ng/ml, (43.04+/-3.06) ng/ml vs (22.15+/-4.34) ng/ml, and (4.78+/-0.70) ng/ml vs (1.61+/-0.34) ng/ml respectively, P less than 0.01], but MMP-9 concentration did not increase [(7.82+/-2.25) ng/ml vs (7.70+/-1.92) ng/ml]. Functional assays in vitro indicated that SMMC-7721 cells after transfection showed higher adhesive, migrant and invasive capabilities than those of the controls (cell adhesion rates were 75.33%+/-10.59% vs 57.34%+/-2.52%; number of outer surface cells in migrant assay was 14.33+/-2.51 vs 6.34+/-1.53; cell number in the invasive assay was 8.23+/-1.53 vs 4.12+/-1.29 respectively, P less than 0.05).</p><p><b>CONCLUSION</b>OPN might enhance the expression of MMP-2 and uPA to promote malignant phenotypes of SMMC-7721 cells.</p>
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Patología
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Transfección
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Activador de Plasminógeno de Tipo Uroquinasa
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Carcinoma Hepatocelular
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Secreciones Corporales
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Metaloproteinasa 2 de la Matriz
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Línea Celular Tumoral
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Osteopontina
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Genética
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Neoplasias Hepáticas
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Hepatology
Año:
2007
Tipo del documento:
Article