Preparation of hemoglobin-loaded nanoparticles and safety evaluation in vitro and in vivo / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 584-588, 2008.
Article
en Zh
| WPRIM
| ID: wpr-291187
Biblioteca responsable:
WPRO
ABSTRACT
Hemoglobin-loaded nano-sized particles with oxygen carrying capacity were prepared. All experiments were performed using biodegradable polymer poly (polyepsilon-caprolactone) (PCL) as matrix polymer. Optimized preparation parameters led to nanoparticles with well-defined characteristics such as size <200 nm, P50 27 mmHg and high encapsulation efficiency up to 99.4%. The results of in vitro and vivo studies suggested that Hb-loaded particles did not activate complements. After the nanoparticles suspension was injected into the mice via tail vein, the particles did not cause significant changes in total platelet counts. Apparently, the hemoglobin-loaded nanoparticles can serve as a potential candidate in substitution for red cells.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Tamaño de la Partícula
/
Poliésteres
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Materiales Biocompatibles
/
Sustitutos Sanguíneos
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Ensayo de Materiales
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Hemoglobinas
/
Química
/
Nanopartículas
Límite:
Animals
Idioma:
Zh
Revista:
Journal of Biomedical Engineering
Año:
2008
Tipo del documento:
Article