Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS / 药学学报
Acta Pharmaceutica Sinica
; (12): 863-866, 2006.
Article
en Zh
| WPRIM
| ID: wpr-294924
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products.</p><p><b>METHODS</b>Optimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra.</p><p><b>RESULTS</b>Elucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples.</p><p><b>CONCLUSION</b>The HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Plantas Medicinales
/
Extractos Vegetales
/
Química
/
Reproducibilidad de los Resultados
/
Cromatografía Liquida
/
Paclitaxel
/
Taxus
/
Espectrometría de Masa por Ionización de Electrospray
/
Espectrometría de Masas en Tándem
/
Métodos
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2006
Tipo del documento:
Article