A novel molecular mechanism of interferon alpha-regulated expression of retinoic acid-induced gene G / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 88-92, 2010.
Article
en Zh
| WPRIM
| ID: wpr-295174
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanisms by which IFN-alpha regulated retinoic acid-induced gene G (RIG-G) expression.</p><p><b>METHODS</b>The expression of STAT1, p-STAT1 and RIG-G in IFN-alpha-treated NB4 cells was detected by Western blot. The roles of STAT1, STAT2 and IRF-9 in IFN-alpha-induced RIG-G expression were analyzed in STAT1-null U3A cells by cell transfection, reporter gene assay, co-immunoprecipitation and chromatin immunoprecipitaion.</p><p><b>RESULTS</b>In U3A cells, only when STAT2 and IRF-9 were co-transfected, the luciferase activities of RIG-G promoter-containing reporter gene could be highly increased about 8-fold compared with that in the control group. Moreover, in the absence of IFN-alpha, similar effects were observed in either IRF-9 co-transfected with wild type or mutant form of STAT2, whereas IFN-alpha could increase the transactivation activity of wild type STAT2 and IRF-9 by 6-fold compared with that without IFN-alpha, but had no effect on mutant STAT2. In addition, STAT2 could interact with IRF-9 and bind to the RIG-G promoter.</p><p><b>CONCLUSION</b>STAT2 may interact with IRF-9 in a STAT1-independent manner. The complex STAT2/IRF-9 is the key factor mediating the expression of RIG-G gene regulated by IFN-alpha. This is a novel signal transduction cascade for IFN which is different from the classical JAK-STAT pathway.</p>
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Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Fosforilación
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Plásmidos
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Transfección
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Leucemia Promielocítica Aguda
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Transducción de Señal
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Regulación Neoplásica de la Expresión Génica
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Interferón-alfa
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Línea Celular Tumoral
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Oncology
Año:
2010
Tipo del documento:
Article