Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the biotransformation of podophyllotoxin by the cell suspension culture and root culture systems of Rheum palmatum.</p><p><b>METHOD</b>Using plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means.</p><p><b>RESULT</b>Cell suspension culture of R. palmatum could convert podophyllotoxin to produce picropodophyllotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyllotoxin to produce epipodophyllotoxin and apopodophyllotoxin.</p><p><b>CONCLUSION</b>Podophyllotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyllotoxin.</p>