Detection and analysis of partial sequences isolated from human and swine in rural area of southern China / 中华流行病学杂志
Chinese Journal of Epidemiology
; (12): 984-987, 2005.
Article
en Zh
| WPRIM
| ID: wpr-295643
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the genotype of swines isolated from humans and their relationships with hepatitis E virus (HEV) in the rural areas of southern China.</p><p><b>METHODS</b>Specimens collected from normal people with HEV-IgM positive, acute hepatitis E patients and from swine in the same area were detected for HEV RNA using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced.</p><p><b>RESULTS</b>13 out of the 132 samples from swine stool, 4 of 26 HEV-IgM positive sera of normal people and 1 of 4 acute hepatitis E patients' stool sample and sera were tested positive for HEV RNA. Data from sequence analysis showed that the identity at nucleotide level was 89.3%-100.0% among the 10 isolates which shared 78.7% - 84.7%, 83.3% - 85.3%, 76.0% - 80.0% and 84.7% - 95.3% nucleotide sequence identity with HEV genotype I, II, III and IV respectively in the region (nt6317- 6466).</p><p><b>CONCLUSION</b>HEV circulating in humans and swine in the area belonged to genotype IV.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Porcinos
/
Enfermedades de los Porcinos
/
Virología
/
Sangre
/
Inmunoglobulina M
/
ARN Viral
/
China
/
Salud Rural
/
Virus de la Hepatitis E
/
Hepatitis E
Tipo de estudio:
Diagnostic_studies
Aspecto:
Determinantes_sociais_saude
Límite:
Animals
/
Humans
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Epidemiology
Año:
2005
Tipo del documento:
Article