Interaction of E3 ligase HUWE1 and eukaryotic translation initiation factor eIF4E / 药学学报
Acta Pharmaceutica Sinica
; (12): 1543-1546, 2014.
Article
en Zh
| WPRIM
| ID: wpr-299100
Biblioteca responsable:
WPRO
ABSTRACT
To explore the regulation of eIF4E, we screened the protein interacting with eIF4E from human cDNA library by using yeast two-hybrid system. Several clones interacting with eIF4E were identified. One of them was homologous with HUWE1 (HECT, UBA and WWE domain containing 1, also named as ARF-BP1, HECTH9 or HUWE1). Cell co-immunoprecipitation showed that eIF4E could bind to HUWE1 in mammalian cells. We also found that HUWE1 bearing the HECT domain is necessary for its association with eIF4E.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Factor 4E Eucariótico de Iniciación
/
Ubiquitina-Proteína Ligasas
/
Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2014
Tipo del documento:
Article