Relationship between M3 receptor and myocyte apoptosis induced by acute myocardial infarction / 药学学报
Acta Pharmaceutica Sinica
; (12): 338-341, 2004.
Article
en Zh
| WPRIM
| ID: wpr-301081
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To explore the effects of M3 receptor on myocyte apoptosis induced by acute myocardial infarction in rats.</p><p><b>METHODS</b>Rat model was induced by ligation of the anterior branch of the left coronary artery. All animals were divided into four groups: sham-operated group, occlusion group, choline group (10 mg x kg(-1), iv), and 4DAMP (4-diphenylacetoxy-N-methylpiperidine-methiodide) group (0.12 mg x kg(-1), iv). The serum malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. The infarct size areas on the myocardium were identified by TTC staining. The apoptosis in cardiomyocyte was detected by TUNEL assay and apoptosis-related proteins in Bcl-2 and Fas expression were measured by immunohistochemistry assay.</p><p><b>RESULTS</b>M3 receptor agonist choline reduced serum MDA content and increased SOD activity. The myocardial expression of Bcl-2 was increased, whereas the expression of Fas was decreased by choline. However, blockade of M3 receptor by 4DAMP completely inhibited these effects of choline on cardiac myocytes.</p><p><b>CONCLUSION</b>Activation of M3 receptor has protective effect on myocyte apoptosis induced by acute myocardial infarction in rat, and this effect might be related to modulating the expression of some immediateearly genes including Bcl-2 and Fas.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Farmacología
/
Piperidinas
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Superóxido Dismutasa
/
Sangre
/
Colina
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Ratas Wistar
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Apoptosis
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Receptores del Factor de Necrosis Tumoral
/
Receptor fas
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2004
Tipo del documento:
Article