Discrimination of Descurainiae Semen and Pantagirus Semen by HPLC fingerprints / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To discriminate Descurainiae Semen and Pantagirus Semen.</p><p><b>METHOD</b>A high-performance liquid chromatographic method was developed to establish the fingerprint of Descurainiae Semen, and hierarchical cluster analysis (HCA) and partial least squares discriminant analysis (PLS-DA) were applied to study HPLC fingerprinting and chemical recognition.</p><p><b>RESULT</b>There exists large difference of chromatographic peaks and its relative peak area of HPLC fingerprints between Descurainiae Semen and Pantagirus Semen, and after conducting statistical analysis, the result demonstrated that all samples were classified into three categories Descurainiae Semen, Pantagirus Semen and their mixtures.</p><p><b>CONCLUSION</b>The developed HPLC fingerprint combined with chemometrics can be utilized to discriminate between Descurainiae Semen and Pantagirus Semen, which was quick, simple, accurate and reliable an can provide the basis for the characterization and quality assess of Descurainiae Semen and Pantagirus Semen.</p>