Peripheral blood T cell subsets and TH1/TH2 cytokines secretion in children with chronic hepatitis C virus infection / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 26-28, 2007.
Article
en Zh
| WPRIM
| ID: wpr-305506
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the cellular immunological abnormalities in children with chronic hepatitis C virus infection.</p><p><b>METHODS</b>(1) The quantity of the peripheral blood T cell subsets in 16 children with chronic hepatitis C virus infection and 10 healthy blood donors was detected by FACS. (2) The levels of the TH1/TH2 cytokines secretion of PBMC in patients and healthy blood donors were detected by ELISA.</p><p><b>RESULTS</b>(1) Compared with normal controls, there was no significant difference in the percentage of CD4+ cells. The percentage of CD8+ cells was significantly higher than that of controls (P < 0.05). The percentage of CD3+ cells was higher and the ratio of CD4+/CD8+ cells was lower, but the difference was not significant (P > 0.05). (2) Compared with the cytokine level of normal control, the levels of IFN-gamma, IL-10 and TNF-alpha notably increased (P < 0.01) while IL-2, IL-4, and IL-12 were not detected in the culture supernatant of PBMCs from both normal control and patients.</p><p><b>CONCLUSION</b>There is an imbalance in peripheral blood T cell subsets and disturbance in cellular immunity in children with chronic hepatitis C virus infection, which may be associated with HCV persistent infection.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Sangre
/
Ensayo de Inmunoadsorción Enzimática
/
Leucocitos Mononucleares
/
Citocinas
/
Subgrupos de Linfocitos T
/
Interleucina-4
/
Interleucina-2
/
Interleucina-10
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Células Th2
/
Células TH1
Límite:
Adolescent
/
Child
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Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2007
Tipo del documento:
Article