Multi-drug resistance and characteristic of integrons in Shigella spp. isolated from China / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 56-61, 2011.
Article
en En
| WPRIM
| ID: wpr-306891
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the characteristic of integrons and the relationship between integrons and antimicrobial resistance in Shigella spp.</p><p><b>METHODS</b>Ninety Shigella strains (83 S. flexneri and 7 S. sonnei) were isolated from the stools of patients in China. Susceptibility to 8 antimicrobials was tested for all isolated strains. PCR, RFLP and sequencing analysis of integrons were applied to all of them.</p><p><b>RESULTS</b>High prevalence of multi-drug resistance (95.6%) was identified. Of the isolates 79 (87.8%) carried integrase genes of class 1 integron (3.3%), class 2 integron (10.0%) or both (74.4%). No intI3 was detected in the tested isolates. The prevalence of intI2 was significantly higher in isolates with multi-drug resistance to at least 3 antibiotics than that in isolates with resistance to 2 and less antibiotics (P<0.05). Gene cassettes dfrA17-aadA5, dfrA12-orfF-aadA2 of class 1 integron and dfrA1-sat1-aadA1 of class 2 integron were identified.</p><p><b>CONCLUSION</b>The class 2 integron may play a role in the emergence of multi-drug resistance in Shigella spp.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Shigella
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Farmacorresistencia Microbiana
/
Secuencia de Bases
/
China
/
Reacción en Cadena de la Polimerasa
/
Cartilla de ADN
/
Resistencia a Múltiples Medicamentos
/
Integrones
/
Genética
Tipo de estudio:
Prognostic_studies
País/Región como asunto:
Asia
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Año:
2011
Tipo del documento:
Article