Experimental study of construction of tissue engineered bone ectopically by human bone marrow mesenchymal stem cells / 中华整形外科杂志
Chinese Journal of Plastic Surgery
; (6): 409-411, 2007.
Article
en Zh
| WPRIM
| ID: wpr-314204
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the possibility and mechanism of construction of tissue engineered bone with human bone marrow mesenchymal stem cells (hBMSCs) as seeding cells and partially demineralized bone matrix (pDBM) as scaffold.</p><p><b>METHODS</b>hBMSCs are cultured and mutiplified. The 4th grade hBMSCs are seeded on the pDBM, the growth and adhesion of hBMSCs on pDBM are observed under scanning electro microscope. The adhesion efficiency is assessed. The complexes are implanted in the nude mice subcutaneously, the pDBM without cells as control. The grafts are taken out on the 8th and 12th week.</p><p><b>RESULTS</b>There is new bone formation on the 8th and 12th week in complex group. There is a layer of osteoblast like cells adhered on the surface of most of the new bone, which suggest the possibility of intramembranous ossification. There is no bone formation in control group.</p><p><b>CONCLUSIONS</b>Tissue engineered bone can be constructed with hBMScs and pDBM in vivo, and the mechanism of which could be intramembranous ossification.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Huesos
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Células de la Médula Ósea
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Adhesión Celular
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Diferenciación Celular
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Biología Celular
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Ingeniería de Tejidos
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Células Madre Mesenquimatosas
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Métodos
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Ratones Desnudos
Límite:
Animals
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Plastic Surgery
Año:
2007
Tipo del documento:
Article