Apoptosis of multiple myeloma cells induced by 8-Cl-cAMP in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1204-1207, 2007.
Article
en Zh
| WPRIM
| ID: wpr-318757
Biblioteca responsable:
WPRO
ABSTRACT
The study was aimed to investigate the possible effects of 8-chloroadenosine 3', 5'-monophosphate (8-Cl-cAMP) on the multiple myeloma cells. The multiple myeloma cell line RPMI8226 was used as in vitro models. The effect on growth inhibition of RPMI8226 cells was evaluated by cell growth and viability curve. DNA fragment was visualized by agarose gel electrophoresis. The amount of apoptosis cells was measured by flow cytometry. Meanwhile Western blot assay were used to detect the change of several key cell cycle regulatory proteins CDK2 and cyclin E in these cells before and after the treatment. The results showed that low dose 8-Cl-cAMP (1-30 micromol/L) inhibited the proliferation and viability of RPMI8226 cells significantly. Agarose gel electrophoresis of DNA revealed the apoptosis characteristic "ladder" pattern. Apoptosis was also confirmed by flow cytometry. In addition, 8-Cl-cAMP was able to inhibit the cell growth through modulating expression of cell cycle regulators CDK2 and cyclin E. It is concluded that 8-cl-cAMP inhibits the proliferation and induce apoptosis of multiple myeloma cells effectively.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Farmacología
/
8-Bromo Monofosfato de Adenosina Cíclica
/
Apoptosis
/
Ciclina E
/
Línea Celular Tumoral
/
Proliferación Celular
/
Quinasa 2 Dependiente de la Ciclina
/
Metabolismo
/
Mieloma Múltiple
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2007
Tipo del documento:
Article