Expression of DNA methylation of APC in peripheral blood and tumor tissue in patients with esophageal squamous cell carcinoma / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 719-722, 2011.
Article
en Zh
| WPRIM
| ID: wpr-321246
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between methylation status of APC gene in both peripheral blood and tumor tissues and clinical-pathology characteristics in patients with esophageal squamous cell carcinoma(ESCC), and to study the dynamic change of APC methylation in peripheral blood in the perioperative period.</p><p><b>METHODS</b>Real-time MSP technique was used to detect methylation status of APC in tumor tissues, adjacent normal tissues and peripheral blood on the day before the surgery, intraoperative, postoperative day 7 in 76 cases with ESCC. Sixty healthy volunteers matched by age and gender were randomly selected as controls.</p><p><b>RESULTS</b>The methylation rate of APC in tumor tissue and peripheral blood was 44.74%(34/76) and 42.11%(32/76), respectively, which were significantly higher than that in adjacent normal tissue and controls [6.58%(5/76) and 1.67%(1/60), P=0.000]. The methylation rates showed good agreement between tumor tissues and peripheral blood, which could be verified by ROC curve(A Zeta=0.849, P=0.000). APC methylation rate was significantly related to pathological staging, lymph node metastasis, depth of invasion, and invasion of nerve and vessel (P<0.05). The results demonstrated that family history of cancer was independently associated with APC methylation in peripheral blood(P<0.05). DNA methylation rates in peripheral blood showed an initial increase and then decreased in the preoperative period, intraoperative and postoperative.</p><p><b>CONCLUSION</b>The methylation rates of APC among free DNA in peripheral blood in patients with ESCC reflect tumor progression, and decrease with the solid tumour resection.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Patología
/
Sangre
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Neoplasias Esofágicas
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Carcinoma de Células Escamosas
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Estudios de Casos y Controles
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Metilación de ADN
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Proteína de la Poliposis Adenomatosa del Colon
/
Metabolismo
Tipo de estudio:
Observational_studies
/
Prognostic_studies
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Risk_factors_studies
Límite:
Adult
/
Aged
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Gastrointestinal Surgery
Año:
2011
Tipo del documento:
Article
Pais de publicación:
CHINA
/
CN
/
REPUBLIC OF CHINA