Recombinant adeno-associated virus vector related impurities / 生物工程学报
Chinese Journal of Biotechnology
; (12): 717-723, 2011.
Article
en Zh
| WPRIM
| ID: wpr-324545
Biblioteca responsable:
WPRO
ABSTRACT
Recombinant adeno-associated virus (rAAV)-based vectors that can stably express therapeutic genes in vivo without detectable side-effect have shown great promise for human gene therapy. A major challenge for translation of promising research to clinical development is how to establish clinically compatible purification methods in separating rAAV from potentially pathogenic impurities, especially rAAV vector-related impurities, a class of impurities corresponding to AAV particles that closely resemble bona fide vectors and are difficult to remove. In this review we summarize the assembly process of rAAV vector-related impurities and their characteristics differed with rAAV vectors, and evaluate several current technologies to prevent their formation or separate them from rAAV stocks.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Fisiología
/
Recombinación Genética
/
Virión
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Replicación Viral
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Terapia Genética
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Dependovirus
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Ensamble de Virus
/
Proteínas de la Cápside
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Vectores Genéticos
/
Genética
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2011
Tipo del documento:
Article