Establishment of a transgenic cell model for preliminary screening of chemopreventive agents / 生物工程学报
Chinese Journal of Biotechnology
; (12): 85-89, 2007.
Article
en Zh
| WPRIM
| ID: wpr-325414
Biblioteca responsable:
WPRO
ABSTRACT
To develop a GFP transgenic cell model under the transcriptional control of TK promoter adjacent to which ARE enhancer was inserted. Synthetic oligonucleotide ARE motif was annealed and purified then inserted into pTK-GFP to construct the vector of pARE-TK-GFP. The TK and ARE-TK fragments were amplified by PCR and cloned into pEGFP-N1 to reconstruct eukaryotic expression vectors of pTK-GFP/Neo and pARE-TK-GFP/Neo. They were transfected into HepG2 cells and clones resistant G418 were isolated. PDTC and Lentinan were used to induce the cell levels of GFP and the fluorescence was measured using a fluorescence plate reader. The results showed that the induced level of GFP is significantly increased and have dose-dependeny in a certain range. This findings indicated that such a cell model offered a potential platform for preliminary screening of all kinds of natural or synthetic chemopreventive agents.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Oligonucleótidos
/
Farmacología
/
Tiocarbamatos
/
Proteínas Recombinantes de Fusión
/
Prolina
/
Ensayos de Selección de Medicamentos Antitumorales
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Gentamicinas
/
Transfección
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Article