Cbl-b and PI3K/Akt pathway are differently involved in oxygen-glucose deprivation preconditioning in PC12 cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 4132-4138, 2013.
Article
en En
| WPRIM
| ID: wpr-327619
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Transient sublethal ischemia is known as ischemic preconditioning, which enables cells and tissues to survive subsequent prolonged lethal ischemic injury. Ischemic preconditioning exerts neuroprotection through phosphatidylinositol 3-kinase (PI3K)/Akt pathway. Cbl-b belongs to the Casitas B-lineage lymphoma (Cbl) family, and it can regulate the cell signal transduction.The roles of ubiquitin ligase Cbl-b and PI3K/Akt pathway and the relationship between them in oxygen-glucose deprivation preconditioning (OGDPC) in PC12 cells were investigated in the present study.</p><p><b>METHODS</b>Oxygen and glucose deprivation (OGD) model in PC12 cells was used in the present study. The 3-(4, 5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, nuclear staining with Hoechst 33258, and Western blotting were applied to explore the roles of Cbl-b and PI3K/Akt pathway and the relationship between them in OGDPC in PC12 cells.</p><p><b>RESULTS</b>Cell viability was significantly changed by OGD and OGDPC. OGD significantly decreased cell viability compared with the control group (P < 0.05), and preconditioning could rescue this damage was demonstrated by the increase of cell viability (P < 0.05). The expression of Cbl-b was significantly increased after OGD treatment. However, the activation of Akt and GSK3β was greatly inhibited. Preconditioning could inhibit the increase of Cbl-b caused by OGD and increase the activation of Akt and GSK3β. LY294002, a specific inhibitor of PI3K, could effectively inhibit the increase of Akt and GSK3β after preconditioning treatment. It partly inhibited the decrease of Cbl-b expression after preconditioning treatment.</p><p><b>CONCLUSION</b>Ubiquitin ligase Cbl-b and PI3K/Akt pathway are differently involved in OGDPC in PC12 cells.</p>
Texto completo:
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Base de datos:
WPRIM
Asunto principal:
Oxígeno
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Fisiología
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Transducción de Señal
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Supervivencia Celular
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Células PC12
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Precondicionamiento Isquémico
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Proteínas Adaptadoras Transductoras de Señales
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Proteínas Proto-Oncogénicas c-cbl
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Proteínas Proto-Oncogénicas c-akt
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Fosfatidilinositol 3-Quinasa
Límite:
Animals
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2013
Tipo del documento:
Article