Cloning and expression of a new glucoamylase gene / 生物工程学报
Chinese Journal of Biotechnology
; (12): 477-524, 2007.
Article
en Zh
| WPRIM
| ID: wpr-328001
Biblioteca responsable:
WPRO
ABSTRACT
According to the reported gene sequence of Rhizopus oryzae glucoamylases, the glucoamylase gene containing four introns was cloned from the total DNA of the natural Rhizopus arrhizu. Specific primers were designed to delete introns by overlapping PCR and a new cDNA sequence of Rhizopus arrhizu glucoamylase was obtained. The accession number in gene bank is DQ903853. This gene is successfully expressed in the Picha pastoris, producing a new protein with a high activity of glucoamylase.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Pichia
/
Rhizopus
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Proteínas Recombinantes
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Proteínas Fúngicas
/
Datos de Secuencia Molecular
/
Regulación Enzimológica de la Expresión Génica
/
Regulación Fúngica de la Expresión Génica
/
Western Blotting
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Glucano 1,4-alfa-Glucosidasa
/
Clonación Molecular
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Año:
2007
Tipo del documento:
Article